Ction with 0.four mM IPTG at 37uC. (B) 1: LMW, 2: purified protein. The
Ction with 0.4 mM IPTG at 37uC. (B) 1: LMW, 2: purified protein. The arrow indicates the purified 14-3-3 recombinant protein. (C) Immunoblot to confirm the reactivity of polyclonal serum…